Unique Cohorts of Salivary Gland Cancer Cells as an in-vitro Model of Circulating Tumor Cells

被引:0
|
作者
Mincy, Callie [1 ,2 ]
Revelt, Luke [1 ]
Carter, Kathryn [1 ]
Reed, Donald [1 ]
Joy, Anita [3 ]
机构
[1] Southern Illinois Univ, Dept Growth Dev & Struct, Sch Dent Med, Alton, IL USA
[2] Southern Illinois Univ, Coll Arts & Sci, Dept Biol Sci, Edwardsville, IL USA
[3] UTHlth Houston, Sch Dent, Dept Diagnost & Biomed Sci, 7500 Cambridge Ave, Houston, TX 77054 USA
来源
关键词
Circulating tumor cells; Circulating tumor microemboli; Salivary gland cancer; Epithelial to mesenchymal transition; Metastasis; E-cadherin; Vimentin; beta-; catenin; alpha-SMA; EPITHELIAL-MESENCHYMAL TRANSITION; SMOOTH MUSCLE ACTIN; WNT/BETA-CATENIN PATHWAY; E-CADHERIN EXPRESSION; BETA-CATENIN; CLINICAL-SIGNIFICANCE; MUCOEPIDERMOID CARCINOMA; PROSTATE-CANCER; METASTASIS; PROGNOSIS;
D O I
10.1007/s12663-024-02250-0
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction The characterization of circulating tumor cells (CTC) and circulating tumor microemboli (CTM) has emerged as both a challenge to the standard view of metastasis, and as a valuable means for understanding genotypic and phenotypic variability shown even within the same cancer type. However, in the case of salivary gland neoplasms, limited data are available for the role that CTCs and CTMs play in metastasis and secondary tumor formation.ru.AQ1 In response to this, we propose that similarities between in vitro clusters of cultured salivary gland cancer cells may act as a surrogate model for in vivo CTCs and CTMs isolated from patients. Materials and Methods Using techniques in immunofluorescence, immunoblotting, and 2-dimensional migration, we isolated and characterized a group of cohort cells from a commercially available cell line (HTB-41). Results: Here, cells exhibited a hybrid phenotype with simultaneous expression of both epithelial and mesenchymal markers (E-cadherin, vimentin, and alpha-SMA). Cohort cells also exhibited increased migration in comparison to parental cells. Conclusion Data suggest that these isolated cell clusters may fucntion as a potential in vitro model of CTCs and CTMs.
引用
收藏
页码:896 / 908
页数:13
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