Bovine serum albumin-imprinted magnetic poly(2-pyrrolidone) microparticles for protein recognition

被引：0
|
作者：
Dencheva, Nadya V. [1 ]
Oliveira, Filipa D. [1 ]
Braz, Joana F. [1 ]
Denchev, Zlatan Z. [1 ]
机构：
[1] IPC – Institute for Polymers and Composites, Department of Polymer Engineering, University of Minho, Guimaraes,4800-056, Portugal
来源：
European Polymer Journal | 2020年 / 122卷
关键词：
This study reports on the synthesis and adsorption properties of molecularly imprinted porous magnetic microparticles (MIP) based on the biodegradable and sustainable poly(2-pyrrolidone) (PPD or PA4). These new PPD MIP materials were obtained via activated anionic ring-opening polymerization of 2-pyrroldone carried out at 40 °C; in the presence of iron fillers and bovine serum albumin (BSA) as a template. Neither solvent; nor additional crosslinking or porogen agents were used in the PPD MIP synthesis. Analogously; PPD particles without BSA imprinting (NIP) were also produced. Depending on the microparticles composition; their yields were in the 55–70 wt% range; the average size varying between 8 and 25 µm. After characterization of the surface topography of all samples; their adsorption capacity toward the BSA target was assessed as a function of the adsorption time; protein concentration and pH of the medium. All three PPD MIP samples displayed adsorption capacity toward BSA being up to one order of magnitude higher as compared to other BSA-imprinted polymer systems. It was found that the rebinding of BSA on MIP is best described by the Langmuir isotherm; whereas for rebinding on NIP the Freundlich isotherm was the more adequate model. On this basis; the nature of the adsorption on MIP and NIP was discussed. The adsorption toward two other proteins; namely Ovalbumin and Cytochrome C was also tested. The newly synthesized BSA-imprinted PPD MIP displayed selective adsorption for the BSA target being dependent on the pH values of the medium. The easy recovery of the Fe-containing MIP and the capacity of all MIP samples for multiple sorption/desorption cycles was demonstrated. © 2019 Elsevier Ltd;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [21] Magnetic composites based on bovine serum albumin and poly(aspartic acid)
    Ghilan, Alina
    Chiriac, Aurica P.
    Nita, Loredana E.
    POLYMER ENGINEERING AND SCIENCE, 2019, 59 (07): : 1409 - 1415
  • [22] Surface protein imprinted magnetic nanoparticles for specific recognition of bovine hemoglobin
    Li, Weiming
    Chen, Miaomiao
    Xiong, Huayu
    Wen, Wei
    He, Hanping
    Zhang, Xiuhua
    Wang, Shengfu
    NEW JOURNAL OF CHEMISTRY, 2016, 40 (01) : 564 - 570
  • [23] Ternary Complexes of Poly(Vinyl Pyrrolidone)-Graft-Poly(2-Dimethylaminoethyl Methacrylate), DNA and Bovine Serum Albumin for Gene Delivery
    Zhang, Biao
    Deng, Liandong
    Xing, Jinfeng
    Yang, Jun
    Dong, Anjie
    JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2013, 24 (01) : 45 - 60
  • [24] Preparation of bovine serum albumin imprinted polymer on silica surface and its molecular recognition function
    Zhou Yanmei
    Xu Wenguo
    Tong Aijun
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 34 (11) : 1551 - 1554
  • [25] Thermo-sensitive surface molecularly imprinted magnetic microspheres based on bio-macromolecules and their specific recognition of bovine serum albumin
    Wang, Yufei
    Ma, Yong
    Zhou, Jingjing
    Su, Kehe
    Zhang, Baoliang
    Zhang, Qiuyu
    JOURNAL OF SEPARATION SCIENCE, 2020, 43 (05) : 996 - 1002
  • [26] A facile synthesis of novel three-dimensional magnetic imprinted polymers for rapid extraction of bovine serum albumin in bovine calf serum
    Liang Yan
    Jing Wang
    Piaopiao Lv
    Dandan Xie
    Zhaohui Zhang
    Analytical and Bioanalytical Chemistry, 2017, 409 : 3453 - 3463
  • [27] A facile synthesis of novel three-dimensional magnetic imprinted polymers for rapid extraction of bovine serum albumin in bovine calf serum
    Yan, Liang
    Wang, Jing
    Lv, Piaopiao
    Xie, Dandan
    Zhang, Zhaohui
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2017, 409 (13) : 3453 - 3463
  • [28] Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition
    Li, Xiangjie
    Zhou, Jingjing
    Tian, Lei
    Li, Wei
    Zhang, Baoliang
    Zhang, Hepeng
    Zhang, Qiuyu
    JOURNAL OF SEPARATION SCIENCE, 2015, 38 (19) : 3477 - 3486
  • [29] Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin
    Yang, Ya-Qiong
    He, Xi-Wen
    Wang, Yi-Zhi
    Li, Wen-You
    Zhang, Yu-Kui
    BIOSENSORS & BIOELECTRONICS, 2014, 54 : 266 - 272
  • [30] Synthesis and characterization of poly(N-vinyl pyrrolidone-alt-maleic anhydride):: Conjugation with bovine serum albumin
    Veron, L
    Revol, M
    Mandrand, B
    Delair, T
    JOURNAL OF APPLIED POLYMER SCIENCE, 2001, 81 (13) : 3327 - 3337
12345