Bovine serum albumin-imprinted magnetic poly(2-pyrrolidone) microparticles for protein recognition

被引：0
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作者：
Dencheva, Nadya V. [1 ]
Oliveira, Filipa D. [1 ]
Braz, Joana F. [1 ]
Denchev, Zlatan Z. [1 ]
机构：
[1] IPC – Institute for Polymers and Composites, Department of Polymer Engineering, University of Minho, Guimaraes,4800-056, Portugal
来源：
European Polymer Journal | 2020年 / 122卷
关键词：
This study reports on the synthesis and adsorption properties of molecularly imprinted porous magnetic microparticles (MIP) based on the biodegradable and sustainable poly(2-pyrrolidone) (PPD or PA4). These new PPD MIP materials were obtained via activated anionic ring-opening polymerization of 2-pyrroldone carried out at 40 °C; in the presence of iron fillers and bovine serum albumin (BSA) as a template. Neither solvent; nor additional crosslinking or porogen agents were used in the PPD MIP synthesis. Analogously; PPD particles without BSA imprinting (NIP) were also produced. Depending on the microparticles composition; their yields were in the 55–70 wt% range; the average size varying between 8 and 25 µm. After characterization of the surface topography of all samples; their adsorption capacity toward the BSA target was assessed as a function of the adsorption time; protein concentration and pH of the medium. All three PPD MIP samples displayed adsorption capacity toward BSA being up to one order of magnitude higher as compared to other BSA-imprinted polymer systems. It was found that the rebinding of BSA on MIP is best described by the Langmuir isotherm; whereas for rebinding on NIP the Freundlich isotherm was the more adequate model. On this basis; the nature of the adsorption on MIP and NIP was discussed. The adsorption toward two other proteins; namely Ovalbumin and Cytochrome C was also tested. The newly synthesized BSA-imprinted PPD MIP displayed selective adsorption for the BSA target being dependent on the pH values of the medium. The easy recovery of the Fe-containing MIP and the capacity of all MIP samples for multiple sorption/desorption cycles was demonstrated. © 2019 Elsevier Ltd;
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