Cleaning protocols in forensic genetic laboratories

被引：1
|
作者：
Kampmann, Marie-Louise [1 ]
Tfelt-Hansen, Jacob [1 ,2 ]
Borsting, Claus [1 ]
机构：
[1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Forens Med, Sect Forens Genet, Copenhagen, Denmark
[2] Copenhagen Univ Hosp, Heart Ctr, Dept Cardiol, Copenhagen, Denmark
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2024年 / 138卷 / 05期
关键词：
Laboratory cleaning; Contamination; DNA; PCR; qPCR; Forensic genetics;
D O I：
10.1007/s00414-024-03232-0
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon (R) removed all amplifiable DNA from the surfaces, whereas DNA AWAY (TM) and the disinfection reagents ethanol, isopropanol, and ChemGene HLD4L did not.
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页码：1787 / 1790
页数：4
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