Identification of MAM1s in Regulation of 3C Glucosinolates Accumulation in Allopolyploid Brassica juncea

被引：2

作者：

Zhangping Li ^{[1
]}

Changtong Zhang ^{[1
]}

Qingze Cai ^{[1
]}

Yulan Zhou ^{[1
]}

Zhongyuan Hu ^{[1
]}

Mingfang Zhang ^{[1
,2
]}

Jinghua Yang ^{[1
,2
]}

机构：

[1] Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University

[2] Key Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture and Rural

来源：

园艺学报(英文) | 2020年 / 6卷 / 06期

关键词：

D O I：

暂无

中图分类号：

S565.4 [油菜籽（芸薹）];

学科分类号：

摘要：

Allopolyploid Brassica juncea is particularly enriched in sinigrin, a kind of 3 C aliphatic glucosinolates(GSLs), giving rise to characteristic taste after picking. However, the molecular mechanism underlying 3 C aliphatic GSLs biosynthesis in this species remains unknown. In this study,we genome-widely identified GSLs metabolic genes, indicating different evolutionary rate of GSLs metabolic genes between subgenomes of B.juncea. Eight methythioalkylmalate synthase(MAMs) homologs were identified from B. juncea, in which six MAM1 s were located in chloroplast and the other two were not detected with any expression. Furthermore, BjMAM1-4, BjMAM1-5, and BjMAM1-6 displayed higher expression levels in leaves than other tissues. Silenced expression analysis revealed that BjMAM1-4 and BjMAM1-6 function in 3 C and 4 C aliphatic GSLs accumulation. The specificity of the substrate selection for the second cycle reaction is much lower than that of the first cycle, suggesting these genes may preferentially catalyze 3 C aliphatic GSLs biosynthesis. Our study provides insights into the molecular mechanism underlying the accumulation of 3 C aliphatic GSLs, thereby facilitating the manipulation of aliphatic GSLs content in B. juncea.

引用

页码：409 / 418

页数：10

共 50 条

[41] Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1
C Rutherford
S Childs
J Ohotski
L McGlynn
M Riddick
S MacFarlane
D Tasker
S Pyne
N J Pyne
J Edwards
T M Palmer
Cell Death & Disease, 2013, 4 : e927 - e927
[42] C-maf, JunB, and Gata3 mediation of IL-4 up-regulation in CD4 T cells from type 1 sphingosine 1-phosphate receptor (S1P1) transgenic (TG) mice
Wang, WG
Huang, MC
Goetzl, EJ
FASEB JOURNAL, 2006, 20 (04): : A482 - A483
[43] Z-DEPENDENCE OF THE DOUBLE CAPTURE IN AQ+ (1S2) + HE,H2 SYSTEMS (A=C,N,O,NE) OBSERVED AT 10 QKEV FOR VARIOUS ANGLES - IDENTIFICATION OF 1S23L3L' LINES - THEORY AND EXPERIMENT
BOUDJEMA, M
BENOITCATTIN, P
BORDENAVEMONTESQUIEU, A
GLEIZES, A
MORETTOCAPELLE, P
CORNILLE, M
DUBAU, J
DOUSSON, S
HITZ, D
JOURNAL DE PHYSIQUE, 1989, 50 (C-1): : 313 - 319
[44] Cloning of Brassica napus phospholipase C2 (BnPLC2), phosphatidylinositol 3-kinase (BnVPS34) and phosphatidylinositol synthase1 (BnPtdIns S1)—comparative analysis of the effect of abiotic stresses on the expression of phosphatidylinositol signal transduction-related genes in B. napus
Shankar Das
Atta Hussain
Cheryl Bock
Wilf A. Keller
Fawzy Georges
Planta, 2005, 220 : 777 - 784
[45] Cloning of Brassica napus phospholipase C2 (BnPLC2), phosphatidylinositol 3-kinase (BnVPS34) and phosphatidylinositol synthase1 (BnPtdIns S1) -: comparative analysis of the effect of abiotic stresses on the expression of phosphatidylinositol signal transduction-related genes in B-napus
Das, S
Hussain, A
Bock, C
Keller, WA
Georges, F
PLANTA, 2005, 220 (05) : 777 - 784
[46] d Identification of two novel mutations inverted right perpendicularP122S (364C>T) and 1601delACinverted left perpendicular in the SLC3A1 gene in type I cystinurics
Gitomer, William L.
Reed, Berenice Y.
Pak, Charles Y. C.
HUMAN MUTATION, 2000, 15 (04) : 390 - +
[47] Gene expression profiling for analysis acquired oxaliplatin resistant factors in human gastric carcinoma TSGH-S3 cells: The role of IL-6 signaling and Nrf2/AKR1C axis identification
Chen, Chih-Cheng
Chu, Chia-Bao
Liu, Ko-Jiunn
Huang, Chi-Ying F.
Chang, Jang-Yang
Pan, Wen-Yu
Chen, Huang-Hui
Cheng, Yun-Hsia
Lee, Kuan-Der
Chen, Miao-Fen
Kuo, Ching-Chuan
Chen, Li-Tzong
BIOCHEMICAL PHARMACOLOGY, 2013, 86 (07) : 872 - 887
[48] sigma-Thienyl complexes of palladium(II), trans-PdBr(C4H4-nBrn-1S-C)(PPh3)(2) (n=1-4) from oxidative addition of bromothiophenes to Pd(PPh3)(4). X-ray structural identification of key intermediates of catalytic debromination of 2-bromothiophene and tetrabromothiophene
Xie, Y
Ng, SC
Wu, BM
Xue, F
Mak, TCW
Hor, TSA
JOURNAL OF ORGANOMETALLIC CHEMISTRY, 1997, 531 (1-2) : 175 - 181
[49] Unconstitutionality of § 19 subparagraph 3 of the Authorization Regulation for Physicians; [Verfassungswidrigkeit von §19 Abs. 3 ärzte-ZV : GG Artt. 12 Abs. 1 S. 1, 19 Abs. 3, 80 Abs. 1 GG; BVerfGG §§31 Abs. 2 S. 2, 93a Abs. 2 lit. b, 93c Abs. 1 S. 1, 95 Abs. 2; SGB V §§95 Abs. 1 S. 2, 95 Abs. 1a S. 1, 95 Abs. 3, 95 Abs. 5, 6 und 7, 96 Abs. 4 S. 2, 98 Abs. 1 und 2, 99 Abs. 1, 100, 101, 103, 104, 126 Abs. 3; RVG §14 Abs. 1, 37 Abs. 2 S. 2, ärzte-ZV §§1 Abs. 3 Nr. 2, 19 Abs. 3]
Kamps H.
Medizinrecht, 2017, 35 (3) : 223 - 227
[50] Identification of 4-(2-(4-amino-1,2,5-oxadiazol-3-yl)-1-ethyl-7-{[(3S)-3-piperidinylmethyl]oxy}-1H-imidazo[4,5-c]pyridin-4-yl)-2-methyl-3-butyn-2-ol (GSK690693), a novel inhibitor of AKT kinase
Heerding, Dirk A.
Rhodes, Nelson
Leber, Jack D.
Clark, Tammy J.
Keenan, Richard M.
Lafrance, Louis V.
Li, Mei
Safonov, Igor G.
Takata, Dennis T.
Venslavsky, Joseph W.
Yamashita, Dennis S.
Choudhry, Anthony E.
Copeland, Robert A.
Lai, Zhihong
Schaber, Michael D.
Tummino, Peter J.
Strum, Susan L.
Wood, Edgar R.
Duckett, Derek R.
Eberwein, Derek
Knick, Victoria B.
Lansing, Timothy J.
McConnell, Randy T.
Zhang, ShuYun
Minthorn, Elisabeth A.
Concha, Nestor O.
Warren, Gregory L.
Kumar, Rakesh
JOURNAL OF MEDICINAL CHEMISTRY, 2008, 51 (18) : 5663 - 5679

← 1 2 3 4 5 →