A CRISPR/Cas12a-based platform for rapid on-site bovine viral diarrhea virus diagnostics

被引:0
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作者
Meixi Wang [1 ,2 ]
Jitao Chang [1 ,3 ]
Yuxin Han [1 ]
Chaonan Wang [1 ]
Songkang Qin [1 ]
Jun Wang [1 ]
Lulu Zhang [4 ]
Yuanmao Zhu [1 ]
Fei Xue [1 ]
Fang Wang [1 ]
Hongliang Chai [2 ]
Yulong Wang [2 ]
Xinjie Wang [4 ]
Xin Yin [1 ]
机构
[1] State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
[2] College of Wildlife and Protected Area, Northeast Forestry University
[3] Institute of Western Agriculture, Chinese Academy of Agricultural Sciences
[4] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural
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中图分类号
S858.23 [牛];
学科分类号
摘要
<正>Bovine viral diarrhea virus(BVDV) is a positive-sense single-stranded RNA virus, belonging to the genus Pestivirus in the Flaviviridae family(Riitho et al. 2020). Currently, BVDV is divided into 3 main genotypes, BVDV-1,BVDV-2, and BVDV-3, based on the genetic differences in the 5′untranslated region(5′UTR)(Muasya et al. 2022). BVDV-1 is the dominant genotype circulating in China(Deng et al. 2020), while BVDV-2 is mainly identified in North America and occasionally in Europe and China(Liu et al. 2012). The BVDV-3 Hobby-like strain has recently been described in Brazil, but the global prevalence has not been investigated yet(Stalder et al. 2005). In addition, the BVDV exists as 2 biotypes: the non-cytopathogenic(NCP) and the cytopathogenic(CP) biotypes.
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页码:2872 / 2876
页数:5
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