An optimized, sensitive and stable reduced graphene oxide–gold nanoparticle-luminol-H2O2 chemiluminescence system and its potential analytical application

The chemiluminescence(CL) performance of luminol is improved using reduced graphene oxide/gold nanoparticle(rGO–AuNP) nano-composites as catalyst. To prepare this catalyst, we propose a linker free, one-step method to insitu synthesize rGO–AuNP nano-composites. Various measurements are utilized to characterize the resulting rGO–AuNP samples, and it is revealed that rGO could improve the stability and conductivity. Furthermore, we investigate the CL signals of luminal catalyzed by rGO–AuNP. Afterwards, the size effect of particle and the assisted enhancement effect of rGO are studied and discussed in detail. Based on the discussion, an optimal, sensitive and stable rGO–AuNP-luminon–H2O2CL system is proposed. Finally, we utilize the system as a sensor to detect hydrogen peroxide and organic compounds containing amino, hydroxyl, or thiol groups. The CL system might provide a more attractive platform for various analytical devices with CL detection in the field of biosensors, bioassays, and immunosensors.