Facile 3D cell culture protocol based on photocurable hydrogels

<正>Introduction Why 3D cell culture urgently needed?Tissue engineering has extremely influenced the development process of basic biological studies and biomedical technology.For quite a long time,the vast majority of these researches have been relying on the experimental results of conventional two-dimensional (2D) cell culture in flask,petri dish or well plate.However,these 2D culture results could be very different with or even totally opposite to the actual situations in vivo where cells grow inside a 3D extracellular matrix (ECM).Therefore,3D cell culture has been playing a more and more significant role because of its higher accuracy and authenticity [1-4].