A New Laccase-Based Biosensor for Epinephrine Determination

被引：0
|
作者：
Arslan, Fatma [1 ]
Durmus, Selma [2 ]
Colak, Ozlem [2 ]
Arslan, Halit [1 ]
机构：
[1] Gazi Univ, Fac Sci, Dept Chem, Ankara, Turkey
[2] Gazi Univ, Inst Sci, Dept Chem, Ankara, Turkey
来源：
GAZI UNIVERSITY JOURNAL OF SCIENCE | 2015年 / 28卷 / 01期
关键词：
Epinephrine; laccase; biosensor; polypyrrole (PPy); polyvinylsulphonate (PVS);
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this paper, a novel amperometric epinephrine (adrenaline) biosensor with immobilization of laccase on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been a complished. Laccase enzyme were immobilized on PPy-PVS film by cross-linking with glutaraldehyde. Determination of epinephrine (EP) was carried out by the reduction of enzymatically produced epinephrinequinone at - 0.220 V vs. Ag/AgCl. The effects of pH and temperature were investigated. There are two linear parts in the region between 0.1 - 1.0 mu M and 1.0 - 10.0 mu M. The storage stability and operation stability of the enzyme electrode were also studied. Interference effects were investigated on response of the biosensor.
引用
收藏
页码：1 / 9
页数：9
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