GAS-PHASE HYDROGEN-DEUTERIUM EXCHANGE AS A MOLECULAR PROBE FOR THE INTERACTION OF METHANOL AND PROTONATED PEPTIDES

被引：112
|
作者：
GARD, E [1 ]
GREEN, MK [1 ]
BREGAR, J [1 ]
LEBRILLA, CB [1 ]
机构：
[1] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 07期
关键词：
D O I：
10.1016/1044-0305(94)85003-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The gas-phase hydrogen/deuterium (H/D) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition, greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.
引用
收藏
页码：623 / 631
页数：9
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