ACTIVE RECOMBINANT C3A OF HUMAN ANAPHYLATOXIN PRODUCED IN ESCHERICHIA-COLI

被引：7
|
作者：
FUKUOKA, Y [1 ]
YASUI, A [1 ]
TACHIBANA, T [1 ]
机构：
[1] TOHOKU UNIV, DEPT PHARMACOL, TB & CANC RES INST, SENDAI, MIYAGI 980, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期
关键词：
D O I：
10.1016/0006-291X(91)91683-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum. © 1991.
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收藏
页码：1131 / 1138
页数：8
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