IMMOBILIZATION OF PENICILLIN-G ACYLASE ON FUNCTIONALIZED MACROPOROUS POLYMER BEADS

被引：33
|
作者：
BAHULEKAR, RV
PRABHUNE, AA
SIVARAMAN, H
PONRATHNAM, S
机构：
[1] NATL CHEM LAB,DIV CHEM ENGN,POONA 411008,MAHARASHTRA,INDIA
[2] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA
来源：
POLYMER | 1993年 / 34卷 / 01期
关键词：
BIOCATALYST ENZYME IMMOBILIZATION; MACROPOROUS SUPPORT; POLYETHYLENEIMINE; PENICILLIN ACYLASE;
D O I：
10.1016/0032-3861(93)90300-Y
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups. Penicillin G acylase (EC 3.5.1.11) was immobilized by covalent binding and the performance of immobilized enzyme was evaluated in batch mode. The expression was estimated as 43%. The shift in pH optima was marginal. The optimum temperature shifted from 40 to 57-degrees-C and K(m) shifted from 31 to 12.8 mumol. Immobilization enhanced the thermal stability of the enzyme.
引用
下载
收藏
页码：163 / 166
页数：4
相关论文
共 50 条
  • [1] THE IMMOBILIZATION OF PENICILLIN-G ACYLASE ON CHITOSAN
    BRAUN, J
    LECHANU, P
    LEGOFFIC, F
    BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (02) : 242 - 246
  • [2] IMMOBILIZATION OF PENICILLIN-G ACYLASE ON METHACRYLATE POLYMERS
    KOILPILLAI, L
    GADRE, RA
    BHATNAGAR, S
    RAMAN, RC
    PONRATHNAM, S
    KUMAR, KK
    AMBEKAR, GR
    SHEWALE, JG
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1990, 49 (02) : 173 - 182
  • [3] Preparation of hydrophilic epoxy polymer magnetic beads and their immobilization for penicillin G acylase
    Key Laboratory of Energy Resources and Chemical Engineering, Ningxia University, Yinchuan 750021, China
    Huagong Xuebao, 2008, 2 (443-449):
  • [4] EFFECT OF MICROENVIRONMENT OF OXIRANE GROUPS ON THE IMMOBILIZATION OF PENICILLIN-G ACYLASE
    BAHULEKAR, RV
    PONRATHNAM, S
    AYYANGAR, NR
    KUMAR, KK
    SHEWALE, JG
    JOURNAL OF APPLIED POLYMER SCIENCE, 1992, 45 (02) : 279 - 284
  • [5] IMMOBILIZATION OF PENICILLIN-G ACYLASE ONTO ALUMINA - EFFECT OF HYDROPHILICITY
    BAHULEKAR, RV
    PONRATHNAM, S
    UPHADE, BS
    AYYANGAR, NR
    KUMAR, KK
    SHEWALE, JG
    BIOTECHNOLOGY TECHNIQUES, 1991, 5 (05) : 401 - 404
  • [6] IMMOBILIZATION-STABILIZATION OF PENICILLIN-G ACYLASE - AN INTEGRATED APPROACH
    GUISAN, JM
    ALVARO, G
    FERNANDEZLAFUENTE, R
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 613 : 552 - 558
  • [7] Influence of activation on the multipoint immobilization of penicillin G acylase on macroporous silica
    Cardias, HCT
    Grininger, CC
    Trevisan, HC
    Guisan, JM
    Giordano, RLC
    BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 1999, 16 (02) : 141 - 148
  • [8] Covalent Immobilization of Penicillin G Acylase onto Grafted Alginate Beads
    Elnashar, Magdy M. M.
    Ali, Ali O.
    Abdallah, Mohga S.
    Hamed, Ragaa R.
    JOURNAL OF COLLOID SCIENCE AND BIOTECHNOLOGY, 2012, 1 (02) : 185 - 191
  • [9] Immobilization of Penicillin G acylase on Amino Functionalized Mesoporous silica
    Sridevi, N.
    Pallavi, Shah
    Asmita, Prabhune
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2008, 3 (04): : 18 - 21
  • [10] Adsorption and immobilization of penicillin acylase on chitosan beads
    Patel, CB
    Gaikar, VG
    SEPARATION SCIENCE AND TECHNOLOGY, 2004, 39 (11) : 2655 - 2675
12345