In planta Agrobacterium-MEDIATED GENETIC TRANSFORMATION IN OKRA {Abelmoschus esculentus (L.) Moench}

被引：4
|
作者：
Menon, Rohit [1 ]
Sarao, Navraj Kaur [1 ]
Pathak, Mamta [2 ]
机构：
[1] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana 141004, Punjab, India
[2] Punjab Agr Univ, Dept Vegetable Sci, Ludhiana 141004, Punjab, India
来源：
APPLIED BIOLOGICAL RESEARCH | 2018年 / 20卷 / 03期
关键词：
Abelmoschus esculentus; Bacillus thuringiensis; shoot and fruit borer; transgenic okra;
D O I：
10.5958/0974-4517.2018.00030.7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In Planta methods have been developed in several plant species to overcome the problems associated with their tissue culture-based transformation. In present study, a method for genetic transformation in okra (Abelmoschus esculentus) was established using in planta Agrobacterium-mediated genetic transformation. The embryos of imbibed seeds pricked from meristematic region of plumule were used as explants for transformation. Agrobacterium tumefaciens strain EHA105 was carrying cry1Ac gene against fruit and shoot borer, CaMV 35S as promoter and nptII as plant selectable marker gene. The putative transgenic plants were confirmed by amplifying transgene through polymerase chain reaction.
引用
收藏
页码：221 / 227
页数：7
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