PURIFICATION AND CHARACTERIZATION OF AN N-ACETYLLACTOSAMINE-SPECIFIC LECTIN FROM TUBERS OF ARUM-MACULATUM

被引：15
|
作者：
ALLEN, AK
机构：
[1] Department of Biochemistry Charing Cross, Westminster Medical School, London, W6 8RF, Fulham Palace Road
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 01期
关键词：
LECTIN; N-ACETYLLACTOSAMINE; (ARUM MACULATUM);
D O I：
10.1016/0304-4165(94)00210-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A lectin was purified from the tubers of Arum maculatum (family Araceae) by affinity chromatography on a thyroglobulin-Sepharose column. The lectin is not a glycoprotein and has a subunit molecular weight of 14 600. It is specifically inhibited by N-acetyllactosamine (Gal beta 1,4GlcNAc), but is not significantly inhibited by monosaccharides or by lactose (Gal beta 1,4Glc), lacto-N-biose 1 (Gal beta 1,3GlcNAc), or chitobiose (GlcNAc beta 1,4GlcNAc). Asialoglycoproteins which contain N-acetyllactosamine structures are even more effective inhibitors of the lectin. This lectin should be a useful probe for N-acetyllactosamine groups in glycoproteins.
引用
收藏
页码：129 / 132
页数：4
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