MAIN STEROLS FROM Ganoderma curtisii AND Ganoderma applanatum FROM HIDALGO STATE, MEXICO

Ganoderma is a group of species of commercial interest and with therapeutic properties. In Mexico there are at least 20 species, including G. curtisii and G. applanatum, which grow in the state of Hidalgo. There are not report on the chemical composition of G. curtisii, while G. applanatum, collected in other countries, has several chemical studies. The aim of this work was to isolate and characterize the main metabolites in these species to contribute to the chemical knowledge of the genus. The separation by chromatographic techniques of the hexane extracts led to the isolation of the known sterols ergosterol, estelasterol, ergosterol peroxide, and 5-aergosta- 7,22-dien-3-one. Their relative proportion in extracts was analysed by HPLC and their characterization was carried out by their physical and spectroscopic properties, mainly by 1D and 2D NMR, and these were compared with described data. These sterols have been reported in Ganoderma, and possess antioxidant, anticancer, antitumor and anti-inflammatory activity.