CIRCULATING HUMAN PREGNANCY-ASSOCIATED PLASMA PROTEIN-A IS DISULFIDE-BRIDGED TO THE PROFORM OF EOSINOPHIL MAJOR BASIC-PROTEIN

被引：1
|
作者：
OXVIG, C
SAND, O
KRISTENSEN, T
GLEICH, GJ
SOTTRUPJENSEN, L
机构：
[1] AARHUS UNIV,DEPT MOLEC BIOL,BLDG 130,DK-8000 AARHUS,DENMARK
[2] AARHUS UNIV,DEPT CHEM,DK-8000 AARHUS,DENMARK
[3] MAYO CLIN & MAYO FDN,DEPT IMMUNOL,ROCHESTER,MN 55905
[4] MAYO CLIN & MAYO FDN,DEPT MED,ROCHESTER,MN 55905
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1993年 / 268卷 / 17期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A previously unrecognized association between pregnancy-associated plasma protein-A (PAPP-A) and the proform of eosinophil major basic protein (proMBP) is demonstrated. PAPP-A isolated from pooled pregnancy serum is shown to be a disulfide-bridged complex with proMBP (PAPP-A/proMBP) in which the subunits of the constituents are present in a 1:1 molar ratio. The results are the outcome of analysis of tryptic and CNBr/tryptic peptides from PAPP-A/ProMBP, sequence analysis of intact and reduced and carboxymethylated PAPP-A/proMBP, and reaction with monoclonal antibodies directed against MBP and its proform. In addition, it is shown that commercial polyclonal anti-PAPP-A is polyspecific, also reacting with MBP.
引用
收藏
页码：12243 / 12246
页数：4
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