METABOLITES FROM A SHAKE CULTURE OF BOTRYTIS-CINEREA

Four new metabolites, 10-oxo-dihydrobotrydial, 4 beta-acetoxy-9 beta-10 beta-15 alpha-trihydroxyprobotrydial, beta-O-methyldihydrobotrydialone and alpha-O-methyldihydrobotrydialone, were isolated from a shake culture of Botrytis cenerea. The second compound, a tricyclic sesquiterpene, is a key biosynthetic intermediate and sheds light on the last steps of the biosynthesis of botrydial derivatives. A higher oxidation level was observed in the metabolites isolated. The structures were elucidated by extensive NMR investigations of the natural compounds and their derivatives.