DIRECT ENANTIOMERIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PROPAFENONE AND ITS MAJOR METABOLITE IN SERUM ON A CELLULOSE TRIS-3,5-DIMETHYLPHENYL CARBAMATE CHIRAL STATIONARY PHASE

被引：13
|
作者：
ABOULENEIN, HY
BAKR, SA
机构：
[1] Drug Development Laboratory, Radionuclide & Cyclotron Operations Department, King Faisal Specialist Hospital and Research Centre, Riyadh, 11211
来源：
BIOMEDICAL CHROMATOGRAPHY | 1993年 / 7卷 / 01期
关键词：
D O I：
10.1002/bmc.1130070110
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A direct, isocratic and simple liquid chromatographic method is described for the enantiomeric separation of propafenone (PRO) and its major metabolite, 5-hydroxypropafenone, using a cellulose tris-3,5-dimethylphenyl carbamate (Chiralcel ODS) column. The stereochemical separation factor (alpha) obtained was 1.14 and the maximum stereochemical resolution factor (R) was 0.80 when using a mobile phase consisting of hexane: 2-propanol: diethylamine (90:10:0.4) at 23-degrees-C. The hydroxy metabolite could not be successfully separated into its corresponding enantiomers under these chromatographic conditions. The method has been used to determine and identify the enantiomers of PRO and its hydroxy metabolite in a serum sample.
引用
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页码：38 / 40
页数：3
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