INSITU MEASUREMENT OF HMG-COA REDUCTASE-ACTIVITY IN DIGITONIN-PERMEABILIZED HEPATOCYTES

被引：14

作者：

GEELEN, MJH ^{[1
]}

PAPIEZ, JS ^{[1
]}

GIRGIS, K ^{[1
]}

GIBSON, DM ^{[1
]}

机构：

[1] INDIANA UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,INDIANAPOLIS,IN 46202

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)81096-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay procedure for HMG-CoA reductase is described which allows rapid measurement of the activity of this enzyme in isolated rat hepatocytes. In a one step procedure digitonin permeabilizes the plasma membrane and at the same time HMG-CoA reductase activity is measured. Digitonin at a concentration of 64 μg per mg of cell protein was found to be optimal for exposing microsomal HMG-CoA reductase to the assay components. The enzyme assay is linear with time up till 5 min and with protein concentrations in the range of 0.06 - 0.6 mg of cell protein per assay. It is shown that cellular enzyme activity is affected by preincubation of intact hepatocytes with a variety of short-term modulators of hepatic cholesterogenesis. © 1991 Academic Press, Inc.

引用

页码：525 / 530

页数：6

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