CLONING AND SEQUENCING OF A GENE ENCODING CARMINOMYCIN 4-O-METHYLTRANSFERASE FROM STREPTOMYCES-PEUCETIUS AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：55
|
作者：
MADDURI, K
TORTI, F
COLOMBO, AL
HUTCHINSON, CR
机构：
[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706
[3] FARMITALIA CARLO ERBA SPA,DIPARTIMENTO BIOTECNOL,UNITA SCREENING,MILAN,ITALY
来源：
JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 12期
关键词：
D O I：
10.1128/JB.175.12.3900-3904.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Sequence analysis of a portion of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a complete open reading frame (dnrK) that showed DNA and protein sequence homology to several O-methyltransferases. Expression of dnrK in Streptomyces lividans and Escherichia coli was done to show that this gene codes for carminomycin 4-O-methyltransferase. The deduced carminomycin 4-O-methyltransferase protein shows a conserved nucleotide binding site for its S-adenosyl-L-methionine cofactor.
引用
收藏
页码：3900 / 3904
页数：5
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