IDENTIFICATION OF BRASSINOLIDE AND CASTASTERONE IN BUCKWHEAT (FAGOPYRUM-ESCULENTUM MOENCH) POLLEN

被引：11
|
作者：
TAKATSUTO, S
OMOTE, K
GAMOH, K
ISHIBASHI, M
机构：
[1] KOCHI UNIV,FAC EDUC,KOCHI 780,JAPAN
[2] NIPPON KAYAKU CO LTD,RES LABS,PHARMACEUT GRP,KITA KU,TOKYO 115,JAPAN
来源：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 03期
关键词：
D O I：
10.1080/00021369.1990.10869991
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
From the pollen of buckwheat (Fagopyrum esculentum Moench), the bioactive substances in a rice lamina inclination test were extracted and partially purified by several chromatographic methods. They were derivatized with 9-phenanthreneboronic acid and dansylaminophenylboronic acid, and then analyzed by reversed-phase high-performance liquid chromatography with fluorimetric detection. The active compounds were identified as brassinolide and castasterone by co-chromatography using authentic samples. In addition, these brassinosteroids were rigorously identified as their bismethaneboronate derivatives by the gas chromatography high resolution-selected ion monitoring method. These data clearly established the presence of brassinolide and castasterone in buckwheat pollen. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.
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页码：757 / 762
页数：6
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