Molecular-genetic Characteristics of Bacteriophage Bacillus cereus FBc - 28 UGSHA

In this article molecular-genetic characteristics of sequenced bacteriophage Bacillus cereus phage FBc - 28 UGSHA is presented. The map of linear DNA with mapping of coding sequences a genome was constructed. According to the known analogues expression products of their genes were determined. It was established that the use of phenol-chlorophorm extraction leads to the best extraction of template DNA. In genome of bacteriophage Bacillus cereus phage FBc - 28 UGSHA the system of molecular-genetic indication (with the use of PCR) of autonomous genetic elements (pathogenic locuses) for specific cereus bacteriophages was developed- candidates for the new high effective phage biopreparation was developed with the aim to enforcement of mediated, allowing to eliminate (destroy) pathogenic microorganisms from edible raw material of animal origin and ready for the use of meat, fish, diary food products. Based on experimental research findings indication of specific fragment of gene HBL enterotoxin with developed systems of oligonucleotides in genomes of Bacillus cereus phage FBc - 28 UGSHA bacteriophage pathogenic locuses were not found.