CHARACTERIZATION OF GLUTAMINE-METABOLISM IN 2 RELATED MURINE HYBRIDOMAS

被引：29
|
作者：
JENKINS, HA
BUTLER, M
DICKSON, AJ
机构：
[1] MANCHESTER POLYTECH, DEPT BIOL SCI, MANCHESTER M15 6BH, LANCS, ENGLAND
[2] UNIV MANCHESTER, DEPT BIOCHEM & MOLEC BIOL, MANCHESTER M13 9PL, LANCS, ENGLAND
来源：
JOURNAL OF BIOTECHNOLOGY | 1992年 / 23卷 / 02期
关键词：
GLUTAMINE; METABOLISM; HYBRIDOMA; MONOCLONAL ANTIBODY; CELL CULTURE;
D O I：
10.1016/0168-1656(92)90090-V
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aspects of glutamine metabolism were examined in two related hybridomas, a high-producing line (PQX B1/2) and a low-producing line (PQX B2/2). The growth and metabolic characteristics of both cell lines were identical or very similar. During batch growth glutamine was completely exhausted from the medium and an examination of the fate of [C-14]glutamine highlighted the importance of this amino acid as an energy source. The relative enzyme activities and the amount of ammonia produced during growth indicated that glutamine is oxidized preferentially via the transmination pathway. The overall rate of glutamine utilization from the growth medium was similar to the rate of [C-14]glutamine uptake which suggests that transport may regulate glutamine metabolism.
引用
收藏
页码：167 / 182
页数：16
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