EXPRESSION OF HUMAN HEPATITIS-B VIRUS SURFACE-ANTIGEN GENE IN TRANSGENIC TOBACCO

被引：0
|
作者：
LIU, YL
WANG, JF
QIU, BS
ZHAO, SZ
TIAN, B
机构：
来源：
SCIENCE IN CHINA SERIES B-CHEMISTRY | 1994年 / 37卷 / 01期
关键词：
HEPATITIS B VIRUS SURFACE ANTIGEN; TRANSGENIC TOBACCO; FOREIGN GENE EXPRESSION;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Expression of Human hepatitis B virus surface antigen (HBsAg) gene in plant was reported for the first lime. The recombinant plasmid pRoKII-HBsAg was constructed by inserting HBsAg gene into the downstream of CaMV 35S promoter of binary vector pRoKII and then introduced into Agrobarteriurn tumefaciens LBA4404. The kanamycin-resistant plants were obtained by Agrobacterium-mediated transformation system. It was shown that HBsAg gene was expressed in transgenic tobacco plants and their progenies by ELISA. The spherical particles of psi 22 nm in the leaf extract of trangenic tobacco were observed by immunosorbent electron microscopy.
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页码：37 / 41
页数：5
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