DEVELOPMENT OF TECHNIQUES TO ANALYZE THE FORMATION OF HIV PROVIRUS IN PRIMARY HUMAN MACROPHAGES

被引：20
|
作者：
COLLIN, M
JAMES, W
GORDON, S
机构：
[1] Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, South Parks Road
来源：
RESEARCH IN VIROLOGY | 1991年 / 142卷 / 2-3期
基金：
英国惠康基金;
关键词：
HIV; MACROPHAGE; PROVIRUS; PCR; DIAGNOSIS; REPLICATION; EARLY STAGE;
D O I：
10.1016/0923-2516(91)90045-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The formation of provirus is an important early event in the life cycle of a retrovirus. We describe a system using PCR for the analysis of this process in primary human macrophages infected in vitro with HIV. We show that products of the first template switch can be detected within 8 h after infection at low multiplicity. The methods described provide a rapid assay of infection with HIV and can be applied in the investigation of a number of aspects of HIV replication in macrophages.
引用
收藏
页码：105 / 112
页数：8
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