A CHIMERIC GENE (ORF256) IS EXPRESSED AS PROTEIN ONLY IN CYTOPLASMIC MALE-STERILE LINES OF WHEAT

被引：87

作者：

SONG, JS ^{[1
]}

HEDGCOTH, C ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI, DEPT BIOCHEM, MANHATTAN, KS 66506 USA

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 01期

关键词：

CYTOPLASMIC MALE STERILITY; COXI; MITOCHONDRIA; MEMBRANE PROTEIN; WHEAT;

D O I：

10.1007/BF00039566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI. Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on western blots of mitochondrial proteins from cytoplasmic male-sterile (ems) wheat (T. aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or ems plants restored to fertility by introduction of nuclear genes for fertility restoration. The 7 kDa protein appears to serve as a marker for ems wheat. Its occurrence as an integral protein of the inner membrane may indicate a ems effect through an influence on mitochondrial membrane function.

引用

页码：535 / 539

页数：5

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