ENZYMATIC DETERMINATION OF N-METHYLCARBAMATE PESTICIDES AT THE NANOMOLAR LEVEL BY THE STOPPED-FLOW TECHNIQUE

被引：11
|
作者：
QUINTERO, MC
SILVA, M
PEREZBENDITO, D
机构：
[1] Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba
来源：
TALANTA | 1991年 / 38卷 / 11期
关键词：
D O I：
10.1016/0039-9140(91)80105-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive and selective kinetic enzymatic method for the determination of N-methylcarbamate pesticides is presented. It is based on their inhibitory effect on electric eel acetylcholinesterase and the use of 5,5'-dithiobis(2-nitrobenzoic) acid (DTNB) as chromogenic reagent for the thiocholine released from the acetylthiocholine iodide substrate. The fast DTNB-thiocholine reaction is monitored photometrically by the stopped-flow technique. Carbaryl, propoxur and carbofuran can be determined at concentrations in the ranges 6.5-120, 2-15 and 0.1-5.0 ng/ml, respectively, by the proposed method. An interference study was also reported.
引用
收藏
页码：1273 / 1277
页数：5
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