QUANTITATION OF HEXADECYLPHOSPHOCHOLINE BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY WITH DENSITOMETRY

被引：17
|
作者：
RUSTENBECK, I
LENZEN, S
机构：
[1] Institute of Pharmacology and Toxicology, University of Göttingen, D-3400 Göttingen
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 525卷 / 01期
关键词：
D O I：
10.1016/S0378-4347(00)83381-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the determination of the antineoplastic ether phospholipid hexadecylphosphocholine (HePC) is presented, based on the separation of the lipids by high-performance thin-layer chromatography charring with a cupric sulphate reagent and quantitation by in situ densitometry. The lower limit of determination is ca. 25 pmol. Concentrated hexane-isopropanol extracts of plasma samples can be applied to the plate without further clean-up, making this method useful for clinical drug monitoring. Additional ion-exchange chromatography and removal of the salt contaminants by gel filtration permits the study of endogenous phospholipids together with HePC from the same sample. © 1990.
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页码：85 / 91
页数：7
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