THE CALMODULIN ANTAGONIST W-7 DEPLETES INTRACELLULAR CALCIUM STORES IN FRTL-5 THYROID-CELLS

被引：15
|
作者：
TORNQUIST, K [1 ]
机构：
[1] UNIV HELSINKI,ENDOCRINE RES LAB,SF-00100 HELSINKI 10,FINLAND
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 01期
关键词：
D O I：
10.1006/bbrc.1993.1007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Incubating Fura 2 loaded thyroid FRTL-5 cells with the calmodulin inhibitor W-7 decreased the ATP-evoked increase in intracellular free calcium. In addition, pretreatment of the cells with W-7 decreased both the thapsigargin-evoked release of sequestered calcium and the entry of extracellular calcium. Studies with 45Ca2+ showed that W-7 decreased the amount of sequestered calcium in the cells. Furthermore, after stimulating with ATP, the ability to sequester calcium was decreased in cells treated with W-7 compared with control cells. The results suggest that calmodulin is necessary for the signal-transduction system in FRTL-5' cells, and may be especially important in maintaining functional intracellular calcium stores in the cells. © 1993 Academic Press, Inc.
引用
收藏
页码：37 / 41
页数：5
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