CHROMOSOMAL LOCALIZATION OF THE HUMAN HISTONE H2A.X GENE TO 11Q23.2-Q23.3 BY FLUORESCENCE IN-SITU HYBRIDIZATION

被引：0
|
作者：
IVANOVA, VS
ZIMONJIC, D
POPESCU, N
BONNER, WM
机构：
[1] NCI,MOLEC PHARMACOL LAB,BETHESDA,MD 20892
[2] NCI,BIOL LAB,BETHESDA,MD 20892
来源：
HUMAN GENETICS | 1994年 / 94卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The human histone H2A.X gene is unusual in that its transcripts are alternatively processed to yield two species, one a 0.6-kb replication-linked histone mRNA and the other a 1.6-kb polyadenylated mRNA. The H2A.X gene has been localized by fluorescence in situ hybridization to chromosome 11q23.2-q23.3, away from the known clusters of human histone genes on chromosomes 1, 6, and 12. Assignment to chromosome 11 was substantiated by analysis of human-hamster somatic cell hybrid lines. As this work was being completed, an 89-bps sequence overlap was found between the downstream regions of the H2A.X gene and the recently sequenced hydroxymethylbilane (HMB)-synthase gene. The H2A.X and HMB-synthase genes have an unusual arrangement, being transcribed towards each other with their polyadenylation sites 330 bp apart. In addition the HMB-synthase gene contains constitutive and erythroid specific promoters. K562, an erythroid cell line, was found to contain a high concentration of the 1.6-kb polyadenylated H2A.X mRNA.
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页码：303 / 306
页数：4
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