AMPLIFIED LUMINOMETRIC ASSAYS OF ALKALINE-PHOSPHATASE USING RIBOFLAVIN PHOSPHATES

被引：7
|
作者：
HARBRON, S [1 ]
EGGELTE, HJ [1 ]
BENSON, SM [1 ]
RABIN, BR [1 ]
机构：
[1] UNIV LONDON UNIV COLL,LONDON WC1E 6BT,ENGLAND
来源：
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1991年 / 6卷 / 04期
关键词：
VIBRIO-HARVEYI; VIBRIO-FISCHERI; NAD(P)H-FMN OXIDOREDUCTASE; ALKALINE PHOSPHATASE;
D O I：
10.1002/bio.1170060407
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An assay for alkaline phosphatase is described which is based on the hydrolysis of riboflavin phosphates (5'FMN or 4'FMN) to produce riboflavin. This is converted to 5'FMN using riboflavin kinase, and then assayed using the bacterial bioluminescent system from Vibrio harveyi or V. fischeri. The most sensitive assay is obtained using 4'FMN, which can measure less than 20 amol after a 1-hour incubation.
引用
收藏
页码：251 / 258
页数：8
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