A CALCIUM-DEPENDENT PROTEIN-KINASE IS PRESENT IN TETRAHYMENA

被引：17
|
作者：
HEGYESI, H
CSABA, G
机构：
[1] Department of Biology, Semmelweis University of Medicine, Budapest
来源：
CELL BIOCHEMISTRY AND FUNCTION | 1994年 / 12卷 / 03期
关键词：
TETRAHYMENA; PROTEIN KINASE; PHYLOGENY; CA-DEPENDENCE;
D O I：
10.1002/cbf.290120311
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A Ca2+-dependent protein kinase of Tetrahymena thermophila has been partially purified and characterized. The molecular mass of the enzyme is less than that of similar enzymes (for example protein kinase C), being about 55 kDa. After purification and in the presence of Ca2+ the enzyme activity increased. The promoter of protein kinase C (PKC) activity, phorbol myristate acetate (PMA), increased the activity while the protein kinase inhibitor H-7 decreased the activity of the enzyme. The experiments demonstrate the presence, activity and similarity to vertebrate enzymes of a protein kinase at a low level of phylogeny.
引用
收藏
页码：221 / 226
页数：6
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