CLONING OF THE SOLE (SOLEA-SENEGALENSIS) GROWTH HORMONE-ENCODING CDNA

被引：15

作者：

PENDON, C

MARTINEZBARBERA, JP

PEREZSANCHEZ, J

RODRIQUEZ, RB

GRENETT, H

VALDIVIA, MM

机构：

[1] UNIV CADIZ,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-11510 PUERTO REAL,SPAIN

[2] CSIC,INST TORRE SAL,CASTELLO DE PLANA,SPAIN

[3] CSIC,INST CIENCIAS MARINAS ANDALUCIA,PUERTO REAL,SPAIN

[4] UNIV ALABAMA,DEPT ORAL BIOL,BIRMINGHAM,AL 35294

来源：

GENE | 1994年 / 145卷 / 02期

关键词：

RECOMBINANT DNA; FISH; EVOLUTION; TELEOST; NORTHERN BLOT; PITUITARY GLAND;

D O I：

10.1016/0378-1119(94)90012-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly(A)(+)RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an insert of 769 nt was found to encode a polypeptide of 203 amino acids (aa), including a signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 17 and 119-nt long, respectively. Northern blot hybridization detected a 0.9-kb RNA species. The sGH cDNA sequence shows homologies of 80.9, 76.9, 73.8 and 64.2% with the GH of tuna, gilthead seabream, flounder and rainbow trout.

引用

页码：237 / 240

页数：4

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