CLONING OF THE SOLE (SOLEA-SENEGALENSIS) GROWTH HORMONE-ENCODING CDNA

被引：15
|
作者：
PENDON, C
MARTINEZBARBERA, JP
PEREZSANCHEZ, J
RODRIQUEZ, RB
GRENETT, H
VALDIVIA, MM
机构：
[1] UNIV CADIZ,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-11510 PUERTO REAL,SPAIN
[2] CSIC,INST TORRE SAL,CASTELLO DE PLANA,SPAIN
[3] CSIC,INST CIENCIAS MARINAS ANDALUCIA,PUERTO REAL,SPAIN
[4] UNIV ALABAMA,DEPT ORAL BIOL,BIRMINGHAM,AL 35294
来源：
GENE | 1994年 / 145卷 / 02期
关键词：
RECOMBINANT DNA; FISH; EVOLUTION; TELEOST; NORTHERN BLOT; PITUITARY GLAND;
D O I：
10.1016/0378-1119(94)90012-4
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly(A)(+)RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an insert of 769 nt was found to encode a polypeptide of 203 amino acids (aa), including a signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 17 and 119-nt long, respectively. Northern blot hybridization detected a 0.9-kb RNA species. The sGH cDNA sequence shows homologies of 80.9, 76.9, 73.8 and 64.2% with the GH of tuna, gilthead seabream, flounder and rainbow trout.
引用
收藏
页码：237 / 240
页数：4
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