COLONY IMMUNOBLOT ASSAY OF BOTULINAL TOXIN

被引：23
|
作者：
GOODNOUGH, MC
HAMMER, B
SUGIYAMA, H
JOHNSON, EA
机构：
[1] UNIV WISCONSIN,DEPT FOOD MICROBIOL & TOXICOL,1925 WILLOW DR,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 07期
关键词：
D O I：
10.1128/AEM.59.7.2339-2342.1993
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.
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页码：2339 / 2342
页数：4
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