IMMUNODETECTION OF AFRICANIZED HONEY-BEE (HYMENOPTERA, APIDAE) SPECIFIC PROTEINS

被引：2
|
作者：
HUNG, ACF
机构：
[1] Bee Research Laboratory, Agricultural Research Service, USDA, Beltsville
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 1992年 / 20卷 / 05期
关键词：
APIS MELLIFERA; IMMUNOBLOTTING; POLYCLONAL ANTISERUM; SUBSPECIES;
D O I：
10.1016/0305-1978(92)90080-W
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four native honey bee proteins (AMP-1, -2, 3, -4) were detected on the IEF gel by the polyclonal antiserum at 1 : 1000 dilution in immunoblotting. AMP 1 and/or AMP-2 were found in all Africanized honey bees (AHB). AMP-3 was found in 88% of AHB. All European honey bees (EHB) had only AMP-4. However, AMP-4 was also found in 53% of AHB. The same antiserum detected three denatured proteins on the SDS-PAGE gel with M(r)s around 39,000, 42,000 and 60,000, All three were found in AHB and EHB.
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页码：395 / 399
页数：5
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