STRAND SPECIFIC PCR AMPLIFICATION OF LOW COPY NUMBER DNA

被引：2

作者：

MEYERHANS, A ^{[1
]}

VARTANIAN, JP ^{[1
]}

WAINHOBSON, S ^{[1
]}

机构：

[1] INST PASTEUR,RETROVIROL MOLEC LAB,28 RUE DR ROUX,F-75724 PARIS 15,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 03期

关键词：

D O I：

10.1093/nar/20.3.521

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the amplification of a single DNA strand at low copy number is described. It is a wholly PCR based approach which involves an initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of primers, one specific for the sequence tagged onto the 5' end of the first round primer, the second specific for the target sequence. Given the protocol used the ratio of the two strands in the final amplification product was 50:1.

引用

页码：521 / 523

页数：3

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