PHYSICOCHEMICAL PRINCIPLES OF CRYOPRESERVATION OF CELL-SUSPENSIONS

被引：0
|
作者：
KORBER, C
机构：
来源：
INFUSIONSTHERAPIE UND TRANSFUSIONSMEDIZIN | 1995年 / 22卷
关键词：
SOLUTE ENRICHMENT; EXOSMOSIS; INTRACELLULAR ICE; OPTIMAL COOLING RATE; CRYOPROTECTANTS;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The basic mechanisms of the freezing of cell suspensions are discussed in terms of solute enrichment and the resulting osmotical consequences. The shrinkage kinetics of the cell volume and the influence of the cooling rate play a crucial role regarding cell damage due to solution effects or intracellular ice formation. The use of cryoadditives permits a substantial reduction of the optimal cooling rate, due to different mechanisms for non-penetrating low molecular weight substances and polymers such as hydroxyethyl starch.
引用
收藏
页码：33 / 35
页数：3
相关论文
共 50 条
  • [41] IRON INDUCTION OF FERRITIN SYNTHESIS IN SOYBEAN CELL-SUSPENSIONS
    PROUDHON, D
    BRIAT, JF
    LESCURE, AM
    PLANT PHYSIOLOGY, 1989, 90 (02) : 586 - 590
  • [42] ACTIVE MAINTENANCE OF CONSTANT BUFFERING CAPACITY IN CELL-SUSPENSIONS
    SIGLER, K
    KNOTKOVA, A
    JANACEK, K
    BIOELECTROCHEMISTRY AND BIOENERGETICS, 1980, 7 (02): : 371 - 375
  • [43] POLAROGRAPHY IN CELL-SUSPENSIONS .2. EXPERIMENTAL RESULTS
    VOIGT, A
    WOLF, H
    STUDIA BIOPHYSICA, 1982, 90 : 235 - 236
  • [44] PURIFICATION OF PLASTID DNA FROM TOBACCO CELL-SUSPENSIONS
    SEYER, P
    HERRMANN, RG
    LESCURE, AM
    PLANT SCIENCE LETTERS, 1982, 25 (03): : 345 - 352
  • [45] ANAEROBIC STRESS IN MEDICAGO-SATIVA CELL-SUSPENSIONS
    LAI, FM
    SENARATNA, T
    MCKERSIE, BD
    JOURNAL OF PLANT PHYSIOLOGY, 1992, 139 (03) : 331 - 338
  • [46] DISPERSION ANALYSIS OF SOME CELL-SUSPENSIONS BY CONDUCTOMETRIC TECHNIQUE
    ALYOSHIN, AM
    LASHMANOV, VI
    TSITOLOGIYA, 1991, 33 (08): : 110 - 112
  • [47] XYLOSE TRANSFER TO LIPIDS BY MEMBRANES OF CARROT CELL-SUSPENSIONS
    QUESADAALLUE, LA
    HAGELIN, K
    LOSCHIAVO, F
    PHYTOCHEMISTRY, 1993, 33 (06) : 1347 - 1352
  • [48] FILTRATION OF WHITE CELL-SUSPENSIONS AT A LOW FLOW STATE
    YAMAGUCHI, S
    NIIMI, H
    BIORHEOLOGY, 1988, 25 (06) : 907 - 907
  • [49] VITRIFICATION OF ORYZA-SATIVA L CELL-SUSPENSIONS
    WATANABE, K
    STEPONKUS, PL
    CRYO-LETTERS, 1995, 16 (05) : 255 - 262
  • [50] POLAROGRAPHY IN CELL-SUSPENSIONS .1. THEORETICAL APPROACH
    VOIGT, A
    DONATH, E
    WOLF, H
    STUDIA BIOPHYSICA, 1982, 90 : 233 - 234
12345