PURIFICATION AND CHARACTERIZATION OF A 43-KDA PROTEASE OF BACTEROIDES-GINGIVALIS

被引：22
|
作者：
FUJIMURA, S
NAKAMURA, T
机构：
[1] Department of Oral Microbiology, Matsumoto
[2] Dental College, Shiojiri, Hiro'oka
来源：
ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 06期
关键词：
Bacteroides gingivalis; protease; protein purification;
D O I：
10.1111/j.1399-302X.1990.tb00441.x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
From the culture supernatant of Bacteroides gingivalis ATCC 33277, a thiol protease was purified to homogeneity by fractional ammonium sulfate precipitation, ion‐exchange chromatography, gel filtration, and isoelectric focusing. Its molecular weight was 43 kDa and showed similar enzymatic properties to a 300‐kDa protease that was previously characterized. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：360 / 362
页数：3
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