PURIFICATION AND STABILIZATION OF PHENYLALANINE AMMONIA-LYASE FROM SPORIDIOBOLUS-PARAROSEUS

被引：5
|
作者：
MONGE, RI
LARA, M
LOPEZMUNGUIA, A
机构：
[1] Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor., 62271
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 06期
关键词：
D O I：
10.1007/BF00160830
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The purification of phenylalanine ammonia-lyase (PAL) from S. pararoseus is described: PAL is recovered from cellular extracts by acid precipitation of contaminating proteins, nucleic acids and carotenoids; concentrated by ultrafiltration and purified by ion exchange chromatography in DEAE-Sephacel, with 80% yield and 32.6 fold purification. Elimination of carotenoids and stabilization with glycerol are introduced in the methodology to increase stability and purification yield.
引用
收藏
页码：423 / 428
页数：6
相关论文
共 50 条
  • [41] PHENYLALANINE AMMONIA-LYASE FROM THE YEAST RHODOTORULA-GLUTINIS
    ABELL, CW
    SHEN, RS
    METHODS IN ENZYMOLOGY, 1987, 142 : 242 - 248
  • [42] PHENYLALANINE AMMONIA-LYASE GENE ORGANIZATION AND STRUCTURE
    CRAMER, CL
    EDWARDS, K
    DRON, M
    LIANG, XW
    DILDINE, SL
    BOLWELL, GP
    DIXON, RA
    LAMB, CJ
    SCHUCH, W
    PLANT MOLECULAR BIOLOGY, 1989, 12 (04) : 367 - 383
  • [43] PHENYLALANINE AMMONIA-LYASE - AN UPDATE ON ITS KINETICS
    KHAN, NU
    BHAT, MA
    VAIDYANATHAN, CS
    CURRENT SCIENCE, 1989, 58 (08): : 427 - 430
  • [44] CHARACTERIZATION OF PHENYLALANINE AMMONIA-LYASE FROM SEVERAL PLANT SPECIES
    JANGAARD, NO
    PHYTOCHEMISTRY, 1974, 13 (09) : 1765 - 1768
  • [45] FURTHER OBSERVATIONS ON PHENYLALANINE AMMONIA-LYASE IN FUNGI
    VANCE, CP
    BANDONI, RJ
    TOWERS, GHN
    PHYTOCHEMISTRY, 1975, 14 (07) : 1513 - 1514
  • [46] ARTIFICIAL CELL MICROENCAPSULATED PHENYLALANINE AMMONIA-LYASE
    BOURGET, L
    CHANG, TMS
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1984, 10 : 57 - 59
  • [47] ISOLATION AND ANALYSIS OF PHENYLALANINE AMMONIA-LYASE GENES FROM TOMATO
    MOUKHAMEDOV, RS
    HEINZ, RA
    ROBB, J
    LEE, SW
    NAZAR, RN
    MOLECULAR BIOLOGY OF THE CELL, 1992, 3 : A82 - A82
  • [48] EFFECTS OF PHENYLALANINE AMMONIA-LYASE ON LEUKEMIC LYMPHOCYTES
    STITH, WJ
    HODGINS, DS
    ABELL, CW
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1972, 13 (MAR): : 92 - &
  • [49] A second form of phenylalanine ammonia-lyase from leaf mustard
    Lim, HW
    Sa, JH
    Park, SS
    Lim, CJ
    MOLECULES AND CELLS, 1998, 8 (03) : 343 - 349
  • [50] PHENYLALANINE AMMONIA-LYASE: AN UPDATE ON ITS KINETICS
    Vaidyanathan, C.S., 1600, Indian Academy of Sciences (58):
12345