PURIFICATION AND STABILIZATION OF PHENYLALANINE AMMONIA-LYASE FROM SPORIDIOBOLUS-PARAROSEUS

被引：5
|
作者：
MONGE, RI
LARA, M
LOPEZMUNGUIA, A
机构：
[1] Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor., 62271
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 06期
关键词：
D O I：
10.1007/BF00160830
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The purification of phenylalanine ammonia-lyase (PAL) from S. pararoseus is described: PAL is recovered from cellular extracts by acid precipitation of contaminating proteins, nucleic acids and carotenoids; concentrated by ultrafiltration and purified by ion exchange chromatography in DEAE-Sephacel, with 80% yield and 32.6 fold purification. Elimination of carotenoids and stabilization with glycerol are introduced in the methodology to increase stability and purification yield.
引用
收藏
页码：423 / 428
页数：6
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