Extracts of thalli of Cetraria alvarensis (Wahlenb.) Vain., C. tilesii Ach., C. juniperina (L.) Ach., C. nivalis (L.) Ach. and C. pinastri (Scop.) S. F. Gray, from several localities in Scandinavia and North America, were subjected to isoelectric focusing and stained for general proteins and for activity of alkaline phosphatase and acid phosphatase. Results of principal component analysis, hierarchical clustering, and cladistic methods show affinities between specimens from the same continent but rather large differences within populations. The differences between American and Scandinavian specimes of C. pinastri suggest a polyphyletic origin of this species.