A NOVEL TERTIARY INTERACTION IN M1 RNA, THE CATALYTIC SUBUNIT OF ESCHERICHIA-COLI RNASE-P

被引:33
|
作者
TALLSJO, A [1 ]
SVARD, SG [1 ]
KUFEL, J [1 ]
KIRSEBOM, LA [1 ]
机构
[1] BIOMED CTR,DEPT MICROBIOL,BOX 581,S-75123 UPPSALA,SWEDEN
关键词
D O I
10.1093/nar/21.17.3927
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phylogenetic covariation of the nucleotides corresponding to the bases at positions 121 and 236 in Escherichia coli RNase P RNA (M1 RNA) has been demonstrated in eubacterial RNase P RNAs. To investigate whether the nucleotides at these positions interact in M1 RNA we introduced base substitutions at either or at both of these positions. Single base substitutions at 121 or at 236 resulted in M1 RNA molecules which did not complement the temperature-sensitive phenotype associated with rnpA49 in vivo whereas wild-type M1 RNA or the double mutant M1 RNA, with restored base-pairing between 121 and 236, did. In addition, wild-type and the double mutant M1 RNA were efficiently cleaved by Pb++ between positions 122 and 123 whereas the rate of this cleavage was significantly reduced for the singly mutated M1 RNA variants. From these data we conclude that the nucleotides at positions 121 and 236 in M1 RNA establish a novel long-range tertiary interaction in M1 RNA. Our results also demonstrated that this interaction is not absolutely required for cleavage in vitro, however, a disruption resulted in a reduction in cleavage efficiency (k(cat)/K(m)), both in the absence and presence of C5.
引用
收藏
页码:3927 / 3933
页数:7
相关论文
共 50 条
  • [31] KINETIC AND THERMODYNAMIC ANALYSIS OF RNA-PROTEIN INTERACTIONS IN THE RNASE-P HOLOENZYME FROM ESCHERICHIA-COLI
    TALBOT, SJ
    ALTMAN, S
    BIOCHEMISTRY, 1994, 33 (06) : 1406 - 1411
  • [32] NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE RNA SUBUNIT (M1 RNA) OF RIBONUCLEASE-P FROM ESCHERICHIA-COLI
    REED, RE
    BAER, MF
    GUERRIERTAKADA, C
    DONISKELLER, H
    ALTMAN, S
    CELL, 1982, 30 (02) : 627 - 636
  • [33] SEVERAL REGIONS OF A TRANSFER-RNA PRECURSOR DETERMINE THE ESCHERICHIA-COLI RNASE-P CLEAVAGE SITE
    SVARD, SG
    KIRSEBOM, LA
    JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (04) : 1019 - 1031
  • [34] SITE-DIRECTED MUTAGENESIS OF M1 RNA, THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P - THE EFFECTS OF AN ADDITION AND SMALL DELETIONS ON CATALYTIC FUNCTION
    LAWRENCE, NP
    ALTMAN, S
    JOURNAL OF MOLECULAR BIOLOGY, 1986, 191 (02) : 163 - 175
  • [35] PRECURSOR OF C4 ANTISENSE RNA OF BACTERIOPHAGES P1 AND P7 IS A SUBSTRATE FOR RNASE-P OF ESCHERICHIA-COLI
    HARTMANN, RK
    HEINRICH, J
    SCHLEGL, J
    SCHUSTER, H
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (13) : 5822 - 5826
  • [36] PRECISE MAPPING OF THE RNPB GENE ENCODING THE RNA COMPONENT OF RNASE-P IN ESCHERICHIA-COLI K-12
    KOMINE, Y
    INOKUCHI, H
    JOURNAL OF BACTERIOLOGY, 1991, 173 (05) : 1813 - 1816
  • [37] SUBSTRATE RECOGNITION BY RNASE-P AND BY THE CATALYTIC M1 RNA - IDENTIFICATION OF POSSIBLE CONTACT POINTS IN PRE-TRANSFER RNAS
    KAHLE, D
    WEHMEYER, U
    KRUPP, G
    EMBO JOURNAL, 1990, 9 (06): : 1929 - 1937
  • [38] Multiple binding modes of substrate to the catalytic RNA subunit of RNase P from Escherichia coli
    Krummel, DAP
    Altman, S
    RNA, 1999, 5 (08) : 1021 - 1033
  • [39] Role of the sequence of the rne-dependent site in 3′ processing of M1 RNA, the catalytic component of Escherichia coli RNase P
    Sim, S
    Kim, S
    Lee, Y
    FEBS LETTERS, 2001, 505 (02): : 291 - 295
  • [40] Dual function of RNase E for control of M1 RNA biosynthesis in Escherichia coli
    Ko, Jae-hyeong
    Han, Kook
    Kim, Yool
    Sim, Soyeong
    Kim, Kwang-Sun
    Lee, Sang-Joon
    Cho, Bongrae
    Lee, Kangryul
    Lee, Younghoon
    BIOCHEMISTRY, 2008, 47 (02) : 762 - 770