The Effects of the Incubation of Hyaluronidase Applicated Oocytes Before ICSI on Fertilization, Embryo Development and Pregnancy Rates

Objective: Intracytoplasmic sperm injection (ICSI) is one of the most important techniques used for the treatment of male infertility. Before ICSI oocytes have to be denuded from cumulus and corona cells using a combination of enzymatic and mechanical methods. In this study 40 IU/ml hyaluronidase has been used to denude the oocytes. The aim of this study is to investigate the effects of the incubation of hyaluronidase applicated oocytes before ICSI on fertilization, embryo development and pregnancy rates. Materials and methods: Patients were randomly selected according to the days of the week. In 114 patient (Group I) oocytes were incubated in 37 C, % 5 CO2 and % 5 O2 for 30-60 minutes before ICSI. In 136 patient (Group II) ICSI is performed immediately after denudation. Results: There was no statistical difference on fertilization rates and number of grade I embryo that were transferred between two groups. Although implantation rates were higher in group II that no incubation had been performed, there was no statistical difference between two groups. Discussion: ICSI can be performed immediately after denudation with hyaluronidase.