INVITRO TRANSCRIPTION AND REPLICATION OF THE MUMPS-VIRUS GENOME

被引：8

作者：

TAKEUCHI, K ^{[1
]}

TANABAYASHI, K ^{[1
]}

OKAZAKI, K ^{[1
]}

HIAHIYAMA, M ^{[1
]}

YAMADA, A ^{[1
]}

机构：

[1] NATL INST HLTH,DEPT MEASLES VIRUS,4-7-1 GAKUEN,MUSASHIMURAYAMA,TOKYO 208,JAPAN

来源：

ARCHIVES OF VIROLOGY | 1993年 / 128卷 / 1-2期

关键词：

D O I：

10.1007/BF01309799

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By the use of lysolecithin-permealized extracts from mumps virus-infected HeLa cells, we have developed an in vitro system, which not only directed the synthesis of mumps virus mRNAs but also supported replication of the genomic RNA. Furthermore, upon transcription of the P gene, both faithful and edited copies of the P gene were detected by RNase mapping with a riboprobe. Thus this system seems to promote biochemical analyses of underlying mechanisms operative in mumps virus gene expression and replication, including RNA editing.

引用

页码：177 / 183

页数：7

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