EFFECT OF H-RAS PROTEINS ON THE ACTIVITY OF POLYPHOSPHOINOSITIDE PHOSPHOLIPASE-C IN HL60 MEMBRANES

被引：7
|
作者：
COCKCROFT, S [1 ]
BARSAGI, D [1 ]
机构：
[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724
来源：
CELLULAR SIGNALLING | 1990年 / 2卷 / 03期
基金：
英国惠康基金;
关键词：
G-protein; GTPγS; H-ras; HL60; membranes; inositol lipids; phospholipase C;
D O I：
10.1016/0898-6568(90)90050-K
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The present study was undertaken to investigate whether purified ras protein can effect the activity of polyphosphoinositide specific phospholipase C in a cell-free membrane system. For this purpose we used homogenous preparations of the proto-oncogenic (H-rasgly12) and the oncogenic (H-rasval12) forms of the human H-ras proteins and membranes prepared from the human leukemic HL60 cells. We demonstrate that both the proto-oncogenic and the oncogenic form of H-ras proteins stimulate phospholipase C activity only when coupled to non-hydrolysable analogues of GTP. © 1990.
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页码：227 / 234
页数：8
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