Phospholipids Isolation from Squid Viscera Residues After Supercritical Carbon Dioxide Extraction

Phospholipids were recovered from squid viscera residues by ethanol extraction after supercritical carbon dioxide(SCO2) extraction and from squid viscera was not processed SCO2 by various organic solvent extraction. SCO2 extraction were performed at 45 degrees C and 20 MPa for removal of non polar lipid molecules from freeze dried squid viscera sample. Phospholipids were extracted from freeze dried squid viscera sample by chloroform, hexane, methanol, and ethanol and from SCO2 extracted squid viscera sample by ethanol. The pH was fixed at 5.7 for all phospholipids extraction conditions. Phospholipid classes were analyzed by HPLC equipped with evaporative light scattering detector (ELSD). Phosphatidyl choline(PC) extracted by ethanol from SCO2 extracted residues was higher than that of extracted by ethanol from squid viscera. But phosphatidyl ethanolamine(PE) and phosphatidic acid(PA) were extracted higher percentage in raw squid viscera. The fatty acid compositions in phospholipids extract by ethanol extract from SCO2 extracted residues were analyzed by gas chromatography(GC). Docosahexanoic acid(DHA) was found in highest percentage in phospholipid extract.