BETA-HYDROXYSTEROID DEHYDROGENASE - ACTIVITY IN MICROEMULSION AND EXTRACTION FROM PSEUDOMONAS-TESTOSTERONI CELLS WITH MICROEMULSION

被引：1
|
作者：
LEE, KM [1 ]
BIELLMANN, JF [1 ]
机构：
[1] UNIV STRASBOURG 1,INST CHIM,CHIM ORGAN BIOL LAB,CNRS,UA 31,1 RUE BLAISE PASCAL,F-67008 STRASBOURG,FRANCE
来源：
BIOCHIMIE | 1990年 / 72卷 / 04期
关键词：
hydroxysteroid dehydrogenase; micelles; microemulsion; Pseudomonastestosteroni; solubilization; stability;
D O I：
10.1016/0300-9084(90)90085-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stability of purified β-hydroxysteroid dehydrogenase activity measured as a function of time was good in buffered cationic and non-ionic microemulsions. The use of 1-pentanol and 1-hexanol in place of 1-butanol as cosurfactant gave increased activity and stability. The NAD+ Michaelis constant was 0.22 mM in buffer and 3.5 mM in waterpool concentratoin in microemulsion. Proteins, among them β-hydroxysteroid dehydrogenase, were extracted from Pseudomonas testosteroni with cationic microemulsion, thus indicating that microemulsions may be utilized in protein release from cells. © 1990.
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页码：285 / 289
页数：5
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