BETA-HYDROXYSTEROID DEHYDROGENASE - ACTIVITY IN MICROEMULSION AND EXTRACTION FROM PSEUDOMONAS-TESTOSTERONI CELLS WITH MICROEMULSION

The stability of purified β-hydroxysteroid dehydrogenase activity measured as a function of time was good in buffered cationic and non-ionic microemulsions. The use of 1-pentanol and 1-hexanol in place of 1-butanol as cosurfactant gave increased activity and stability. The NAD+ Michaelis constant was 0.22 mM in buffer and 3.5 mM in waterpool concentratoin in microemulsion. Proteins, among them β-hydroxysteroid dehydrogenase, were extracted from Pseudomonas testosteroni with cationic microemulsion, thus indicating that microemulsions may be utilized in protein release from cells. © 1990.