ACTIVATION OF NOCICEPTIVE REFLEXES BY PERIPHERAL KAINATE RECEPTORS
被引:0
|
作者:
AULT, B
论文数: 0引用数: 0
h-index: 0
AULT, B
HILDEBRAND, LM
论文数: 0引用数: 0
h-index: 0
HILDEBRAND, LM
机构:
来源:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
|
1993年
/
265卷
/
02期
关键词:
D O I:
暂无
中图分类号:
R9 [药学];
学科分类号:
1007 ;
摘要:
The rat isolated spinal cord-tail preparation was used to examine the ability of peripherally applied excitatory amino acids to stimulate ventral root reflexes. Bolus applications (3 sec) of domoate (0.1-10 muM) or kainate (KA; 10-300 muM) to exposed skin of the rat tail stimulated ventral root responses comparably to those evoked by capsaicin (0.3-10 muM) or bradykinin (0.1-10 muM). The ventral root potential evoked by both capsaicin and KA is thought to be a nociceptive response because both compounds selectively activate peripheral C-fibers. L-Glutamate (0.01-10 mM), quisqualate (0.1-1 mM) and amino-5-methyl-4-isoxazole-propionic acid (0.1-1.0 mM) were of lower potency than KA, whereas N-methyl-D-aspartate (1 mM) and L-aspartate (10 mM) were inactive. Responses evoked by prolonged application of KA faded rapidly. Capsaicin-evoked responses partially faded during prolonged application, but residual spiking activity was recorded for at least 30 min. KA did not evoke any observable response during application of capsaicin. The effects of KA and capsaicin were blocked by spinal application of morphine (0.3-1.0 muM) in a naloxone-reversible manner, consistent with activation of peripheral nociceptive afferents. The action of KA, but not capsaicin, was competitively inhibited by the amino-5-methyl-4-isoxazole-propionic acid/KA receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (10-100 muM) applied to the rat tail (pA2 = 5.9). Conversely, ruthenium red (1 muM) selectively blocked capsaicin-evoked nociceptive reflexes. The existence of functional KA receptors on nociceptive afferents suggests that peripherally released glutamate or other excitatory amino acids could be involved in nociception or neurogenic inflammation.
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Tai, Changfeng
Larson, Jeffrey A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Larson, Jeffrey A.
Ogagan, P. Dafe
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Ogagan, P. Dafe
Chen, Guoqing
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Capital Med Univ, Sch Rehabil Med, China Rehabil Res Ctr, Dept Urol, Beijing, Peoples R ChinaUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Chen, Guoqing
Shen, Bing
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Shen, Bing
Wang, Jicheng
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Wang, Jicheng
Roppolo, James R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
Roppolo, James R.
de Groat, William C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Dept Urol, Pittsburgh, PA 15213 USA
机构:
Univ Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
IRCCS Neuromed, Pozzilli, IS, ItalyUniv Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Serrao, Mariano
Coppola, Gianluca
论文数: 0引用数: 0
h-index: 0
机构:
GB Bietti Eye Fdn IRCCS, Dept Neurophysiol Vis & Neurophthalmol, Rome, ItalyUniv Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Coppola, Gianluca
Di Lorenzo, Cherubino
论文数: 0引用数: 0
h-index: 0
机构:
Univ Roma La Sapienza, ICOT, UCADH, I-04100 Latina, ItalyUniv Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Di Lorenzo, Cherubino
Di Fabio, Roberto
论文数: 0引用数: 0
h-index: 0
机构:Univ Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Di Fabio, Roberto
Padua, Luca
论文数: 0引用数: 0
h-index: 0
机构:Univ Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Padua, Luca
Sandrini, Giorgio
论文数: 0引用数: 0
h-index: 0
机构:
IRCCS C Mondino Fdn, UCADH, Pavia, ItalyUniv Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy
Sandrini, Giorgio
Pierelli, Francesco
论文数: 0引用数: 0
h-index: 0
机构:
Univ Roma La Sapienza, ICOT, UCADH, I-04100 Latina, ItalyUniv Roma La Sapienza, UOC Neurorehabil, I-04100 Latina, Italy